top of page

Mammalian Cell Combinatorial Library

Picture1.jpg

Directed evolution is an effective way of protein engineering and typically performed in microbial systems. Even mammalian cells support protein folding, proper post-translational modifications, and cellular signaling all critical for studying mammalian biology and developing therapeutics, conducting directed evolution in mammalian cells is largely handicapped due to lack of a robust method to construct high quality libraries. We develop platform technologies that meet all desired criteria of an ideal library construction in mammalian cells – single variant per cell, accurate genomic integration, uniform transcription level, and large diversity (Nucleic Acid Res, 2023; Front. Bioeng. Biotechnol., 2024). We expect our methods have broad applications for functional biopharmaceutical development in mammalian cells.

bottom of page