Mammalian Cell Combinatorial Library

Directed evolution is an effective way of protein engineering and typically performed in microbial systems. Even mammalian cells support protein folding, proper post-translational modifications, and cellular signaling all critical for studying mammalian biology and developing therapeutics, conducting directed evolution in mammalian cells is largely handicapped due to lack of a robust method to construct high quality libraries. We develop platform technologies that meet all desired criteria of an ideal library construction in mammalian cells – single variant per cell, accurate genomic integration, uniform transcription level, and large diversity. We expect our methods have broad applications for functional biopharmaceutical development in mammalian cells.